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Bangladesh Journal of Biochemistry. Vol. 1, No. 1, 1-8,
June 1995
Tumour vaccine activity of 64KDa Stress protein derived from myocobacterium
bovis bcg
Jin-ichi SASAKI,
Department of Bacteriology, Hirosaki University School of Medicine,
Hirosaki 036, Japan
Kris HUYGEN, Michel DEJEHANSART,
Institute Pasteur du Brabant, Brussels, Belgium.
Chowdhury R.AHSAN, Tetsutaroh ONO,
Department of Bacteriology, Hirosaki University School of Medicine,
Hirosaki 036, Japan.
and Jacqueline De BRUYN
Institute Pasteur du Brabant, Brussels, Belgium.
(Received October 17, 1994)
Abstract
Experiment
was carried out to examine the tumour vaccine activity of Mycobacterium
bovis BCG HSP64 in Meth A tumour model of BALB/c mouse. This antigen
was broadly distributed among animal and human types of tumor cells
likely to be a tumor-associated antigen. Mice were immunized with
BCG HSP64 (10 mg
per mouse) three times at one week interval in advance. Meth A tumor
cells were intradermally transplanted. The vaccinated mice inhibited
tumor growth at 30%, indicating the induction of tumor protective
immunity. The increasing production of antibody was observed in serum
at high level of titer (104-105 dilution
times) in vaccinated mice. Further, the levels of cytokines such as IL-2,IL-6,IFN-g,or
TNF-a
were also elevated in spleen cell culture supernatants
or peritoneally exudated cells originated from BCG HSP64 vaccinated
mice in response to BCG HSP64 stimulant. These results suggests that
the BCG HSP64 possibly possesses tumour vaccine activity by enhancing
production of antibody and cellular immunity.
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Bangladesh
Journal of
Biochemistry. Vol. 1, No. 1, 9-15, June 1995
CHOLESTEROL LOWERING EFFECT OF HILSHA FISH OIL IN FEMALE RATS
Sushil SINHA and Rafiqur RAHMAN
Department of Biochemistry, University of Dhaka, Dhaka-1000, Bangladesh.
(Received November 13, 1994)
Abstract
The body
oil of hilsa (Tenualosa ilisha), a fatty riverine tasty fish of Bangladesh,
when fed to hypercholesterolemic female rats, dramatically reduced
their serum cholesterol level. The oil feeding had significantly reduced
both serum VLDL and LDL-bound cholesterol while elevating the HDL-bound
cholesterol. Consistent with this, the HDL-LDL ratio which was depressed
by cholesterol feeding, was restored by oil feeding.
Assay of the hepatic HMG-CoA reductase, the rate limiting enzyme of
cholesterol biosynthesis, revealed that oil feeding had significantly
depressed its activity manifested by a relatively higher HMG-CoA/MVA
ratios found in the livers of the oil-fed animal. These results indicate
that one mechanism by which hilsa oil reduces blood cholesterol is
by inhibiting endogenous synthesis of cholesterol.
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Bangladesh
Journal of
Biochemistry. Vol. 1, No. 1, 17-22, June 1995
INTRINSIC RESISTANCE OF LEUKEMIA CELLS TO AN ANTICANCER DRUG
M. Perwaiz IQBAL
Department of Biochemistry, The Aga Khan University, Stadium Road,
Karachi-74800, Pakistan.
(Received December 13, 1994)
Abstract
A number of mechanism
have been shown to be operative in mammalian cells for the development
of resistance to the anticancer drug, methotrexate. Recently, there
have been reports of the intrinsic resistance of certain leukemia
cells to methotrexate. Using L1210 leukemia cells, we have shown that
this intrinsic resistance to methotrexate is due to a form of dihydrofolate
reductase having low affinity for this drug.
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Bangladesh
Journal of
Biochemistry. Vol. 1, No. 1, 23-32, June 1995
TOTAL WHITE BLOOD CELL COUNT AND PHAGOCYTIC ACTIVITY OF THE NEUTROPHILS
IN MONKEYS WITH EXPERIMENTAL SHIGELLOSIS
Anwarul FERDOUS and Laila N.
ISLAM
Department of Biochemistry, University of Dhaka, Dhaka-1000, Bangladesh.
(Received
January 10, 1995)
Abstract
The effect
of experimental shigellosis upon the total white blood cell count
and the phagocytic activity of neutrophils was studied in monkeys
on different days after oral challenge with Shigellae. Fifteen monkeys
were first challenged with S. dysenteriae type 1 which caused
shigellosis in nine monkeys. After recovery, the monkeys were challenged
again with S. flexneri 2a. There were marked changes in the
sensitivity in the animals and also in the phagocytic activity of
their neutrophils during shigellosis compared to unchallenged controls.
However, no difference in the effects was observed in the monkeys
during shigellosis caused by either S. dysenteriae 1 or S.
flexneri 2a. The WBC count decreased slightly within the first
week of challenge, increased during the second and third weeks but
ultimately returned to
the normal level while the animals recovered. The proportion of neutrophils
phagocytosing opsonized yeast varied between 68 and 96% but that for
unopsonized yeast were 2-8%, in normal
healthy monkeys. The corresponding values were 96-99% and 12-50%,
respectively on day 6 of challenge. Also, the number of opsonized
yeast phagocytosed by one hundred neutrophils was 446-796 in normal
healthy monkeys but 900-980 in challenged monkeys on day 6. Neutrophils
from monkeys ill with shigellosis were found to be highly activated
as they phagocytosed more yeast particles.
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Bangladesh
Journal of
Biochemistry. Vol. 1, No. 1, 33-40, June 1995
DEGRADATION OF SOLUBLE SERUM PROTEINS IN HEVEA BRASILIENSIS
LATEX BY BOTTOM FRACTION ACID PROTEASE
Kamruzzaman AMPON, Abdullah SIPAT
and Mohd. Arif SYED
Department of Biochemistry and Microbiology,
Faculty of Science and Environmental Studies, Universiti Pertanian
Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia.
(Received October 17, 1994)
Abstract
The degradation
of soluble serum protein mixtures by proteases that are present in
the bottom fraction lutoids of Hevea brasiliensis latex was investigated
to assess their possible involvement in in vitro protein turnover.
The degradation showed a pH optimum of about 3.5. The strong inhibition
by pepstatin suggested that acid protease may be the major proteolytic
enzyme involved. The serum proteins were degraded less rapidly after
denaturation compared to the native state. There was a good correlation
of digestibility with the molecular sizes of the protein substrates
used.
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Bangladesh
Journal of
Biochemistry. Vol. 1, No. 1, 41-46, June 1995
ISOLATION AND PURIFICATION OF LECTIN FROM GARDEN PEA ( PISUM SATIVUM
L.)
M. Shamsul HOQUE, Balaram ROY, M. M.
NURUDDIN and M. Abdul HAMID
Department of Agricultural Chemistry, Hajee Mohammad Danesh Krishi
College, Dinajpur, Bangladesh.
(Received October 10. 1994)
Abstract
A method is
described for the isolation and purification of a lectin from garden
pea (Pisum sativum) by affinity chromatography on Sephadex
G-150 column. The purified lectin has an apparent molecular weight
of 49,000. It showed haemagglutination (2% erythrocytes) at a concentration
of less than 4 mg/ml
and caused 100% mortality of the larvae of Callosobruchus maculatus
at a concentration of 2.5%.
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Bangladesh
Journal of Biochemistry. Vol. 1, No. 1, 47-52, June 1995
FORMATION OF HISTAMINE IN CHLORPROMAZINE TREATED GUINEA-PIGS
B. H. RIDZWAN
Department of Biomedical, Faculty of Allied Health Sciences, Universiti
Kebangsaan Malaysia, 50300 Kuala Lumpur, Malaysia.
(Received November 2, 1994)
Abstract
Oral administration
of 14C-histidine
induced the presence of 14C-histamine
in certain tissues of both control and chlorpromazine (CPZ)-treated
(30 mg/kg body wt., i. p.) guinea-pigs. The mean 14C-histamine
contents in the tissues of the CPZ-treated animals were found slightly
lower than in the control animals. However, the amounts formed were
very small in comparison to the endogenous histamine contents of the
tissues.14C-histidine
induced the presence of 14C-histamine
in certain tissues of both control and chlorpromazine (CPZ)-treated
(30 mg/kg body wt., i. p.) guinea-pigs. The mean 14C-histamine
contents in the tissues of the CPZ-treated animals were found slightly
lower than in the control animals. However, the amounts formed were
very small in comparison to the endogenous histamine contents of the
tissues.
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